The following experiment investigates the activity of an determines the effect o

Question

The following experiment investigates the activity of an determines the effect of the varying the enzyme concentration. Peroxidase catalysis the breakdown of hydrogen peroxide with the production of water and oxygen. Certain dyes will go from a colorless to colored form upon oxidation, a property that allows detection of peroxidase activity. In the presence of hydrogen peroxide and peroxidase, orthophenylene diamine (OPD) will be changed from a colorless dye to a yellow compound as it is oxidized Using a spectrophotometer, you quantitated the enzyme reaction by measuring the absorbency from the oxidized OPD at 410 nm. The more colored OPD produces in a given time period, the faster the enzyme reaction. The following are the contents of experimental test tubes (Table 1) and the absorbance readings: Write a hypothesis for this experiment What is the substrate of the enzyme peroxidase in this experiment? Which test tube contains the experiment designed as "no enzyme control" (see Table 1)

Explanation / Answer

A. The hypothesis behind this is :- The peroxidase catalyzes the breakdown of hydrogen peroxide with water & oxygen, in the presence of peroxidase enzyme.

The OPD dye also changes from colourless to a yellow oxidised form, it means that the more coloured OPD produced in a given time period , the faster the enzymatic reaction.

B. HYDROGEN PEROXIDE is the Substrate.

C. Test Tube Named as "BLANK"

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