For what is co-immunoprecipitation used? a. to determine if a protein-of-interes

Question

For what is co-immunoprecipitation used? a. to determine if a protein-of-interest binds to a specific DNA sequence b. to examine protein-protein interactions in the nucleus instead of in the cytoplasm c. to examine protein-protein interactions in the cytoplasm instead of the nucleus d. to allow a protein to be expressed in mammalian cell culture e. none of the above What is a problem associated with immuno-based arrays? a. Proteins that are bound to solid supports may not be representative of intracellular conditions. b. The antibody may cross-react with other cellular proteins, producing a false positive. c. Low concentrations of some proteins may not be able to compete for active sites compared to those that are in abundance. d. Proteins that are often found in complexes may have the antibody binding site masked by the other proteins in the complex. e. All of the above are problems associated with immuno-based arrays. Which of the following has been extensively studied using protein interaction arrays? a. proteins in yeast that bind calmodulin or phospholipids b. proteins in yeast that bind to glutathione-S-transferase c. proteins that are able to bind to biotin and streptavidin d. proteins that are able to bind to various cofactors present in the sample e. none of the above. Which of the following methods is the best way to analyze a metabolome? a. high-pressure liquid chromatography b. mass spectroscopy c. nuclear magnetic resonance d. thin layer chromatography e. ELISA Which of the following would be the best method to identify thousands of different proteins simultaneously? a. MALDI-TOF b. traditional MS c. SILAC d. MuDPIT e. SELDI

Explanation / Answer

12. A

CHIP is technique technique to determine the protein DNA interaction which follows the principle of Co-immunoprecipitation.

13. E

These all problems are associated with Immuno based arrays.

14. C

Biotin and strepovidin used in various immunoprecipitation.

15. D

Two dimentional Thin Layer Chromatography used to Comparative MetabolomeProfiling.

16. C

SILAC-based quantitative proteomics (SILAQ) is an innovative technology used in high throughput quantitative analysis of large protein complexes, protein-protein and protein-small molecule interactions.

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