1. Identify the gene from which the query sequence originates (Name of gene) 2.
ID: 223964 • Letter: 1
Question
1. Identify the gene from which the query sequence originates (Name of gene)
2. Provide the FULL protein sequence encoded by the gene.
3. Are different splice variants known for this gene?
4. What human disease has been connected to this gene?
5. Calculate molecular weight (kiloDalton, kD) and calculated pI (the pH where the protein carries no net electrical charge) of the protein.
6. Provide the reference (in proper reference form: Author; Year; Title; Journal Name; Volume; Page Numbers) for a recent publication involving the identified gene. This reference should NOT be a web page reference.
7. Are there homologues for the identified gene in other systems? Identify one homologue in a invertebrate system (if there is none, provide a vertebrate homologue).
8. What is the function (e.g. transcriptional regulation, transmembrane signaling, kinase, protease etc.) of the protein(s) encoded by the gene.
9. Generate a FULL protein sequence alignment for one of the identified putative protein products with at least one similar invertebrate protein (if there is none, use a vertebrate homologue).
10. Generate a secondary structure prediction for one identified protein.
Here is the sequence: TGGGCGAAGGGCCACTGTCCCTTCATGCTG
Here is a website: http://www.ncbi.nlm.nih.gov
Please explain how to get the answer for each question. Please don't just give me the answer. Thanks!
Explanation / Answer
1. Based on BLAST results the the query sequnce is originated from Human ORFeome Gateway entry vector pENTR223-ALAS2. LT742273.1
2. Open reading frames are highlighted in red. Please select one of the following frames
5'3' Frame 1
W A K G H C P F Met L
G R R A T V P S C
G E G P L S L H A
Q H E G T V A L R P
S Met K G Q W P F A
A Stop R D S G P S P
5. Theoretical pI/Mw: 6.92 / 2508.90
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