1. Identify the gene from which the query sequence originates (Name of gene) 2.
ID: 215261 • Letter: 1
Question
1. Identify the gene from which the query sequence originates (Name of gene)
2. Provide the full ... (2 bookmarks) 1. Identify the gene from which the query sequence originates (Name of gene) 2. Provide the full protein sequence encoded by the gene (single letter amino acid abbreviation form).
3. Are different splice variants known for this gene?
4. What human disease has been connected to this gene?
5. Calculate molecular weight (kiloDalton [kD]) and the calculated pI of the protein (the pH where the protein carries no net electrical charge).
6. Provide the reference (in proper reference form: Author; Year; Title; Journal Name; Volume; Page Numbers) for a recent publication involving the identified gene. This reference should NOT be a reference to a web page.
7. Are there homologues for the identified gene in other systems? Identify one homologue in an invertebrate system (if there is none, provide a vertebrate homologue).
8. What is the function (e.g. transcriptional regulation, transmembrane signaling, kinase, protease etc.) of the protein(s) encoded by the gene.
*9. Generate an protein sequence alignment for one of the identified putative protein products with at least one similar invertebrate protein (if there is none, use a vertebrate homologue).
*10. Generate a secondary structure prediction for one identified protein.
Here is the sequence: GCCTACCGGAGACATGAGAAGAAGAGGAAG
http://www.ncbi.nlm.nih.gov is a website you can use to begin. Please explain to me how you got the answer for each question. I NEED THIS ASAP!!! THANK YOU!!!!!!
Explanation / Answer
1) Gap junction beta-2 protein (GJB2)
2) FASTA Format :-
>sp|P29033|1-226
MDWGTLQTILGGVNKHSTSIGKIWLTVLFIFRIMILVVAAKEVWGDEQADFVCNTLQPGC
KNVCYDHYFPISHIRLWALQLIFVSTPALLVAMHVAYRRHEKKRKFIKGEIKSEFKDIEE
IKTQKVRIEGSLWWTYTSSIFFRVIFEAAFMYVFYVMYDGFSMQRLVKCNAWPCPNTVDC
FVSRPTEKTVFTVFMIAVSGICILLNVTELCYLLIRYCSGKSKKPV
3) The gene contains 2 distinct gt-ag introns. Transcription produces 3 different mRNAs, 2 alternatively spliced variants and 1 unspliced form. There are 2 probable alternative promotors and 4 validated, alternative polyadenylation sites.
4) Deafness, Disease mutation, Ectodermal dysplasia, Ichthyosis, Non-syndromic deafness, Palmoplantar keratoderma
5) MW. 26kDa
isoelectric point :- 8.13
6)
"Transcriptional downregulation of gap-junction proteins blocks junctional communication in human mammary tumor cell lines."
Lee S.W., Tomasetto C., Paul D., Keyomarsi K., Sager R.
J. Cell Biol. 118:1213-1221(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
7). No.
Pan Troglodytes.
8). Functions:-
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