In an experiment similar to the Lederberg and Tatum experiment, researchers grew

Question

In an experiment similar to the Lederberg and Tatum experiment, researchers grew two strains of E. coli in complete medium. E. coli strain Ax has the genotype Amp^s bio^+ trp^+ leu-. E. coli strain AY has the genotype Amp^R bio^- trp^- leu^+. The researchers then plated the bacteria on different media types. Next, the researchers mixed equal amounts of the AX and AY E. coli strains. on which type(s) of media would growth provide evidence that genetic transfer had occurred between the two strains of bacteria? Select all that apply.

Explanation / Answer

As per the growth of both the strains is considered, the mixture of AX and Ay styrains will grow on bio - leu - trp-.

This is because first strain is wild for bio and trp. it means strain AX can grow in the media that lacks bio and tro but cannot grow in the media lacking leu as it is not wild for leu.

While tghe other strain can grow in the media lacking leu, because this strain is not wild for leu. But cannot grow in the media lacking trp and bio.

Therefore if the genetic transfer has occured successfully, then strain AX will be able to grow in the media lacking a particular nutrient. If gene transfer take place, the wild type gene for a nutrient will be transferred to the strains' DNA.

Therefore the correct answer will be bio- trp-leu-.

Related Questions

Navigate

• Browse (All)
• Browse I
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.