______ RFLP ______ A, T, G, C ______ PCR ______ primers ______ Denaturation ____
ID: 222769 • Letter: #
Question
______ RFLP ______ A, T, G, C ______ PCR ______ primers ______ Denaturation ______ Anneal ______ Extend ______ STRs ______ mitochondrial DNA ______ nuclear DNA ______ 46 ______ nucleotides ______ length polymorphisms Common source of voluntary DNA ______ samples ______ Chlorox ______ Phosphate groups ______ TAE buffer ______ Taq polymerase ______ Dr. Fisher ______ DNA degraders a. Cheek cells b. Nucleotides found in DNA c. Cleaner that removes DNA well d. heat DNA to 94C e. enzyme used in PCR f. Warmth, Moisture, sunlight, time g. polymerase chain reaction h. Found in more copies than nuclear DNA i. short tandem repeats j. allow primers to stick to DNA k. Restriction fragment length polymorphism l. Made UP of a phosphate, base, and a sugar m. number of chromosomes in human nucleus n. amplified by the 13 CODIS STR primer sets o. 72C, nucleotides are added to DNA p. Responsible for negative charge on DNA molecules q. STRs can form these by having varying numbers of repeated sequences r. your favorite Forensics instructor s. used in solution for running gel electrophoresis t. Anneal to the template in PCRExplanation / Answer
1)Restriction fragment length polymorphisms - RFLP
2) A T G C - Nucleotides found in DNA
3) PCR- polymerase chain reaction
4) Anneal to the template in PCR- primers
5) Heat DNA to 94degree C - denaturation
6) Allow primers to stick to DNA - anneal
7)72 C nucleotides added to DNA- extend
8) Short tandem repeats- STR s
9)Found in more copies than nuclear DNA- mitochondrial DNA
10) Amplified by the 13 CODIS primer dataset - nuclear DNA
11) 46- Number of chromosomes found in human nucleus
12) Made up of phosphate base and sugar - nucleotides
13) STRs can form these by varying number of repeated sequences- length polymorphisms
14) Cheek cells- common source of voluntary DNA samples
15 Chlorox- Cleaner that removes DNA well
16) Responsible for negative charge on DNA- phosphate groups
17)TAE buffer - used in solution for running gel electrophoresis
18 Enzyme used in PCR- Taq polymerase
19) Dr. Fisher - Your favourite forensics instructor
20) Warmth, moisture, sunlight, time - DNA degraders
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