You have identified a protein factor that when added to in vitro protein synthes

Question

You have identified a protein factor that when added to in vitro protein synthesis reaction increases the incorporation of 35S-methionine from 4 pmoles/minute to 8 moles/mean. When you centrifuge these reactions and measure polyribosomes and free ribosomes, you observe that more of the ribosomes are present as polyribosomes for the reactions that contain your stimulatory factor. Which explanation is consistent with this result? a. Your factor binds to the small subunit to promote translation initiation b. Your factor binds to the large subunit to promote translation termination c. Your factor binds to the ribosome to decrease the rate of elongation d. Your factor binds to the ribosome to increase the rate of elongation

please explain answer

Explanation / Answer

Answer - Option A - The factor binds to the small subunit to promote translation initiation. It is because, it is also involved in 35S- methionine incorporation and it has poly ribosomes, which means both initiation and elongation has occurred and hence the answer is option A.

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