A scientist created a mutation in the gene that encodes the Cactus protein to ma

Question

A scientist created a mutation in the gene that encodes the Cactus protein to make a Cactus protein that cannot be phosphorylated by Tube/Pelle (Mutant A). They also created a mutation that made a Cactus protein that is unable to bind to the Dorsal protein (Mutant B). Unfortunately, they weren't paying attention one day and forgot to label the vials containing the mutant flies. To solve the mystery of which vial contained mutant A flies and which vial contained mutant B files, they decide to perform in situ hybridization for snail expression. Describe the pattern of gene expression of snail that would be expected for these two mutants, explaining why the mutations in the Cactus protein would cause this expression pattern.

Explanation / Answer

Ans.

the vial for which is snail expression is very low contains the mutant A flies.

because the cactus protein in mutant A flies cannot be phosphorylated by Tube/Pelle, cactus will not be degraded and will continuously be bound to Dorsal. due to which dorsal will not be able to enter the nucleus resulting in a absence of gradient. as snail is activated by peak levels of dorsal gradient, snail will not be expressed in this case.

the vial for which is snail expression is high contains the mutant B flies.

as cactus is unable to bind to the dorsal protein, dorsal enters the nucleus and induces snail expression.

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