8 Which of the following is an advantage of proteins produced by senetic enginee

Question

8 Which of the following is an advantage of proteins produced by senetic engineering? a. high purity b batch to batch consistency C, high specific activity d. all of the above A potential host-related contaminant in recombinant protein praduct derived from bacterial host is Denatured protein Growth medium components Column materials b. c. d. Endotoxins les of PCR, how 10 If you start with one double-stranded DNA molecule and you perform SIX cyc many double-stranded copies of the DNA will you have? a. 6 c. 16 d. 32 e. 64 onto which are spotted hundreds of thousands of tiny drops of DNA that 11 Small solid supports can be used to screen gene expression. a. DNA microarrays b. cloning library C. Southern blot d. autoradiographs is a method of separating proteins by their sedimentation properties 12 a. b. Centrifugation Chromatography Filtration d. Electrophoresis 13 Separation of proteins by polyacrylamide gel electrophoresis PAGE) is based on a. Shape b. C. d. size Charge All of the above Which of the following is an example of post-trancriptional modification challenge faced while 14 producing recombinant protein in microbes? a. Splicing b. Glycosylation C. Proteolytic degradation d. Inclusion body formation

Explanation / Answer

8. D. All of the above.

9. B. Growth medium contaminants .

10. E. 64.

In each cycle of PCR one strand acts as template and forms new DNA molecule. Thus after 6 cycles, 64 DNA molecules will be formed.

11. A. DNA microarrays.

DNA microarray is the collection of small DNA spots attached to solid surface.

12. B. Centrifugation.

In centrifugation, particles are departed depending upon their sedimentation coefficient.

13. C. Charge.

PAGE electrophoresis is t3chnique of operating protiens based on their electrophoretic (charge) properties.

14. A. Splicing.

Related Questions

Navigate

• Browse (All)
• Browse 8
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.