5 and 6. Choose from the following to answer questions A) BLAST or Basic Local A
ID: 218664 • Letter: 5
Question
5 and 6. Choose from the following to answer questions A) BLAST or Basic Local Alignment Search Tool B) Mass spectrometry C) Transcriptomics D) Multiple sequence alignment E) PubMed 5. A scientist has recently sequenced a gene and she is interested in knowing whether it is to C also present in other organisms. She would start by searching GenBank with identify homologs 6. Having determined that indeed homologs of the gene are present in GenBank, the next logical step is to search E to obtain published literature on the gene of interest. 7. The 5'-end of a polynucleotide chain contains the chemical group A) Amino group B) Carboxyl group C) Hydroxyl group D) Carbonyl group E) Phosphate group 8. The RNA polymerase binds to which region of prokaryotic genes to initiate transcription? A) Operator sequence B) Termination signal Promoter ) Poly A tail E) Enhancer region 9. DNA polymerases do not initiate DNA synthesis de novo, i.e., without a priming end. Priming in vivo is achieved by synthesis of a short fragment of removed by theactivity of DNA polymerase I in prokaryotes. , which is later A) RNA, 5' to 3'exonuclease B) DNA, 3'to 5' exonuclease C) Protein, 5' to 3' endonuclease D) RNA, 3' to 5' endonucleaseExplanation / Answer
5) A - Blast
The Blast tool is used to identify homologs of genes in other organisms.
6) E - Pubmed
Pubmend is a database of all published scientific literature.
7) E - Phosphate group.
The 5' end of a polynucleotide chain such as DNA or RNA contains a phosphate group where as the 3' end contains a hydroxyl group.
8)C- promoter
RNA polymerase binds to the promoter region to initiate transcription of a prokaryotic gene.
9) A - RNA , 5' to 3' exonuclase
Lagging stand is synthesised in short segments. primers of RNA are used to prime the synthesis of the lagging strand.
After complete synthesis of the strand the RNA primers are removed by exonuclease.
10 ) DNA ligase
11) DNA primase
12) DNA polymerase I
During synthesis of Lagging strand, DNA primase adds the RNA primers that are required for priming of DNA synthesis. After the synthesis of the new DNA on th lagging strand, RNA primers are removed by DNA polymerase I , the gaps are filled and the short DNA fragments are linked by DNA ligase.
Therefore , if short DNA fragments accumilate, there is a mutation in DNA ligase and hence short DNA fragment accumilate. Similarly, DNA primase is essential for addition of RNA primers during replication and DNA polymerase I is required to remove the RNA primers.
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