You have the shown DNA strand. The restriction enzyme Mscl recognizes the consen

Question

You have the shown DNA strand. The restriction enzyme Mscl recognizes the consensus sequence, TGG CCA. There is a second allele which contains a SNP at the location of the star. You design a probe to determine the presence of either allele for several individuals. The problem location is indicated by the box saying probe. In the gel image, indicate which of the following genotypes each individual is using one of the following descriptors: homozygous allele 1, homozygous allele 2, or heterozygous. 01 Allele 1 Probe TGG CCA TGG CCA TGG CCA 100bp 500bp 750bp Probe TGG CCA TGA CCA TGG CCA Allele 2 800bp 600bp 400bp 200bp | 100bp

Explanation / Answer

The Mscl restriction endonuclease recognized the consensus sequence of TGG CCA. If we observe allele one there are three cutting site for Mscl endonucelase, one at 100 bp, 500bp and at 750bp which gives a fragment of 400bp and 250bp. In allele 2 only two cutting site for Mscl enzyme are present at 100bp and 750bp only. So it gives rise to 650bp (750bp – 100bp) fragment only. Because one SNP is present at 500bp site of Mscl (instead of TGG CCA, it is TGA CCA).

So lane 1 and 4 having 650bp fragment is an allele 2, lane 2 and 5 having 250bp fragment is an allele 1 and lane 3 is heterozygous for both alleles.

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