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Bacteria Pathogen Assignment Three ( Chapter 10 Classification) After studying C

ID: 218063 • Letter: B

Question

Bacteria Pathogen Assignment Three ( Chapter 10 Classification) After studying Chapter 10. Please post the following information about your pathogen (the one you have been studying all semester) First include an image of your pathogen again with its correct name(1 point) 1. Give it full Taxonomic hierarchy classification. You can google this or use Bergey's Manual (Appendix F in Textbook 12 ed). Make sure to use the correct spelling. (5 points) Domain (Bacteria) Everybody same here as no Archae are pathogens . No Kingdoms in Bacteria and Archae only Eukaryotes Phyla Class Order . Family Genus (Italics for genus and species) Species (remember to use lower case for species) 2. Find three ways that your pathogen is identified for clinical purposes of identification. Be specific in why that identification is used. Example: if using the acid fast stain then mention that your pathogen has mycolic acid in its cell wall. OR If using the ELISA TEST then explain what the antibody in the ELISA test is targeting.. (pathogen protein or an antibody in the patients serum) (There are many identification methods discussed in Chapter 10) (3 points) 3. Comment on three other students identification techniques. (How is their pathogen similar and how is it different) 2 points per comment (6 points total) ( do not use the classification from first part of assignment for discussion. Submit to the discussion Copy and paste questions for easier identification.

Explanation / Answer

A common pathogen used in laboratory is E. coli. The taxonomical hierarchy is as follows:

Domain: Bacteria

Phylum: Proteobacteria

Class: Gammaproteobacteria

Order: Enterobacteriales

Family: Enterobacteriaceae

Genus: Escherichia

Species: coli

The following tests are done to identify E.coli

1.       Gram staining: A positive sample appears gram Negative having a rod shape, with variable capsule.

2.       Under confocal microscope, the bacteria appears as rod shaped motile bacteria with flagella.

3.       IMViC tests will have the following result: Indole: Positive, Methyl-Red: Positive, Voges-Proskauer test: Negative, Citrate test: Negative

4.       Catalase test is positive, while oxidase test is Negative

5.       Oxidative fermentation with gas formation is observed