ding ONLY the luminal domain of US3 was fused in-frame to Figure 1. The DNA enco
ID: 217985 • Letter: D
Question
ding ONLY the luminal domain of US3 was fused in-frame to Figure 1. The DNA enco the DNA that codes for GFP (green fluorescent prot this fragment, called A128GFP, localized to the ER(F determine which amino acids in the luminal domai ure 1, panel B below). To retention/retrieval sequence, the US3 sequence in each fusion con progressively shortened at the 3'-end to produce fusion proteins that contained less and less C-terminal sequence from US3. Constructs are named for the amino acids they contain. For instance, A128GFP contains amino acids 1-128 of US3 and ?84GFP contains amino acids 1-84. The localization of each of these truncated fusion proteins was examined in cells. When translated in the cell n carry the ER struct was 5. Is this a find it, lose it and/or move it experiment? Explain your choice. TM(20aa) CTSaa US3 GFP 4128GFP A74GFP C A7OGFP 465GFP 455GFP 450GFP 438GFP 484GFP 0 A38GFP FIG. 1. Essential domain for ER retention of the US3 glycoprotein. (A) Schematic diagram of the chimeric constructs with the GFP reporter and US3 luminal domain used to identify the retention elements. TM, transmembrane domain; CT, cytoplasmic tail. (B) HtTa cells expressing the indicated mutants were analyzed for intracellular localization by immunonoorescence microscopy s Similas resulas were obtained in at least three 6. (1pt) Fig. 1 - Which US3-GFP (1pt) Fig. 1 - What US3 amino acid sequences contain the ER retention/retrieval sequences? a. aa. 129-161 b. aa. 85-128 c. aa. 55-65 d. aa. 51-55 e. aa. 39-50 f. aa. 191-205 7. fusion constructs do NOT localize to the ER? Choose any that apply a. A84GFP b. ?65GFP c. ?70GFP d. A55GFP e. A38GFPExplanation / Answer
Answer 5) In the given experiment, we are trying to lose the amino acid sequence responsible for ER retention/retrieval so that we do not observe ER retention of the truncated fusion protein if we lose the ER retention sequence in the mutant construct.
It means, it must be a "Lose it experiment", where we are trying to lose the ER retention/retrieval sequence from the mutant construct.
Answer 6 d) ?55GFP e) ?38GFP
Reason- As observed in the pictures, we do not see ER localization in the ?55GFP, ?50GFP ?38GFP. therefore, option d) and e) and correct from the given options.
Answer 7 c) aa. 55-65
Reason- We see the ER localization up to ?65GFP, it means that the ER retention/retrieval sequence must be present within 1-65 amino acids. but, we do not see ER localization in ?55GFP which indicates that we lost the ER retention signal beyond 1-55 amino acids. considering both the deduced facts, we can conclude that the ER retention/retrieval sequence must be present within the 55-65 US3 amino acids.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.