In vitro transcription using a DNA template and T7 RNA polymerase is a useful me

Question

In vitro transcription using a DNA template and T7 RNA polymerase is a useful method to produce RNA. The DNA template contains a promoter region and a template region. For example, the following DNA will yield an mRNA transcript of sequence 5’GCGAUUUCUGA3’:

5’ TAATACGACTCACTATA 3’

3’ ATTATGCTGAGTGATATCGCTAAAGACT 5’

The underlined region corresponds to the template from which the RNA will be synthesized (CGCTAAAGACT) (note this should be the underlined region). The duplex region before that constitutes the promoter for T7 RNA polymerase. For each new RNA sequence, a new DNA template must be synthesized.

Given the DNA template sequence and the sequence of the T7 RNA polymerase promoter (shown above), what is the sequence of the RNA we are analyzing?

5’TAATACGACTCACTATAGGGCTTCGTTAGGTGAGGCTCCTGTATGGAGATACGCTGCTGCCCAA AAATGTCCAAAGACGCCAATGGGTCAACAGAAATCATCGACATAAGGTGATTTTTAATGCAGCT GGATGCTTGTCCTATGCCATACAGTGCTAAAGCTCTACGATTGAAGCCCA3’

Explanation / Answer

5’TAATACGACTCACTATAGGGCTTCGTTAGGTGAGGCTCCTGTATGGAGATACGCTGCTGCCCAA AAATGTCCAAAGACGCCAATGGGTCAACAGAAATCATCGACATAAGGTGATTTTTAATGCAGCT GGATGCTTGTCCTATGCCATACAGTGCTAAAGCTCTACGATTGAAGCCCA3’

This is the sequence of DNA WHICH WILL BE TRANSCRIBED.

RNA SEQUENCE FORMED WILL BE

GCUGAGUGAUAUCCCGAAGCAAUCCACUCCGAGGACAUACCUCUAUGCGACGACGGGUU

UUUACAGGUUUCUGCGGUUACCCAGUUGUCUUUAGUAGCUGUAUUCCACUAAAAACGUCGA

CCUACGAACAGGAUACGGUAUGUCACGAUUUCGAGAUGCUAACUUCGGGU

Related Questions

Navigate

• Browse (All)
• Browse I
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.