34. This type of medium was used to prepare tubes not primarily uued for the cul
ID: 217415 • Letter: 3
Question
34. This type of medium was used to prepare tubes not primarily uued for the culhtivalion af to determine the oxygen requirements of different bacteria. a. Fluid thioglycollate medium b. TGY A shake ekes c. Brewer's agar d.Nutrient agar 35. A solid medium used n lab to culture anaerobes in petri dishes contaising thiodyolas, socing to culture agent, and resazurin, an a. Fluid thioglycollate medium b. Brewer's agar oxidati indicator is c. Trypticase soy agar d. All of the above 36. The most common cause of a urinary tract infection is a. E.coli b. S aureus c. P. vulgaris d. B. cereus 37. Which of the following tubes represents the growth of a facultative organiom suck as E 2 3 4 5 a. Tube 1 b. Tube 2 Tube 3 Tube 4 38. Natural antimicrobial agents produced by bacteria and fungi are a) Endospores b) Antibiotics c) Antibodies d) All of the above 39. A test used for measuring the effectiveness of certain antimicrobials against athogens is the a. Dormer method b. Kirby Bauer methocd c. Streak plate method d. None of the above 40. Which antibiotic below is the most effective sgaint the patsogem growing on the prtri dExplanation / Answer
Answer 34. Medium required to determine the oxygen requirements of different bacteria is Fluid thioglycollate medium. Thiglycollate have strong reducing properties and autoclaving the medium removes most of the oxygen present in the medium. When becteria are cultured in the medium oxygen slowly diffuses in the medium and bacterial density in the medium increases in the area where oxygen concentration is most optium for the growth of that particular bacteria.
Answer 35. The correct option is Fluid thioglycollate medium. Resazurin is present in the medium as an indicator of oxidation. Whenever oxidation happens pink coliur will be generated in the test tube at the site of oxidation.
Answer 36. Most common cause of urinary tract infection is E. coli. Urinary tract infection occurs when bacteria from outside enter the urinary tract through urethra.
Answer 37. Right option for this question will be Tube 3. Facultative anaerobes like E.coli thrive in oxygen rich conditions but can also grow in absence of oxygen by relying on fermentation and anaerobic respiration. Therefore, there will be rich growth in top position but growth will also be present throughout the tube.
Answer 38. Natural antimicrobial agents produced by bacteria and fungi are Antibiotics. Penicillin produced by penicillium fungi and streptomycin from Streptomyces griseus are the most commonly used antibiotics.
Answer 39. The answer for this question is Kirby Bauer method. It is also known as disk diffusion method. This method uses antibiotic containing disks attached on the bacterial culture plates to test which antibiotic is able to inhibit the growth of that particular bacteria.
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