Sequences of single strands of DNA are shown below for two different pieces of D
ID: 216832 • Letter: S
Question
Sequences of single strands of DNA are shown below for two different pieces of DNA. Assume that the DNA sequences are not at the beginning of a gene and that the spaces between each DNA triplet represent the correct reading frame. Label all sequences 5’ and 3’, where appropriate. Keep everything aligned properly.
Synthesize the complementary DNA sequence.
B. Transcribe the mRNA sequence starting with the 5’ end on the left-hand side.
C. Write the complementary tRNA sequence for each reading frame.
D. Use the genetic code table from our lecture notes or from our textbook (Bauman, 4th ed., p. 208; Bauman, 3rd ed, p. 209; Bauman, 2nd ed., p. 211) to translate the amino acid sequence of the encoded polypeptide, using the three-letter code for each amino acid. Note: Use only the 20 amino acids that are most commonly found in all living organisms.
3’ 5’
1. CCC GAC TCA CTT TAG CCG TTA TAG GGA AAA ATC
A.
B.
C.
D.
5’ 3’
2. GGT GCG CAC ATT TAC AAA GTT AAC CTC CAG GTA
A.
B.
C.
D.
(micro biology assignment)
Explanation / Answer
In DNA A binds with T and G binds with C or vice versa. When coming to RNA A codes to U, G codes to C, C codes to G and T codes to A.
So, the answers are
Sequence 1
3'CCC GAC TCA CTT TAG CCG TTA TAG GGA AAA ATC5' DNA
5'GGG CTG AGT GAA ATC GGC AAT ATC CCT TTT TAG3' DNA
5'GGG CUG AGU GAA AUC GGC AAU AUC CCU UUU UAG3' mRNA
3'CCC GAC UCA CUU UAG CCG UUA UAG GGA AAA AUC5' tRNA
N'Pro Asp Ser Leu End Pro Leu End Gly Lys IleC' Peptide
Sequence 2
3'GGT GCG CAC ATT TAC AAA GTT AAC CTC CAG GTA5' DNA
5'CCA CGC GTG TAA ATG TTT CAA TTG GAG GTC CAT3' DNA
5'CCA CGC GUG UAA AUG UUU CAA UUG GAG GUC CAU3' mRNA
3'GGU GCG CAC AUU UAC AAA GUU AAC CUC CAG GUA5' tRNA
N'Gly Ala His Ile Tyr Lys Val Asn Leu Gln ValC' Peptide
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