Sequences of single strands of DNA are shown below for two different pieces of D
ID: 216813 • Letter: S
Question
Sequences of single strands of DNA are shown below for two different pieces of DNA. Assume that the DNA sequences are not at the beginning of a gene and that the spaces between each DNA triplet represent the correct reading frame. Label all sequences 5’ and 3’, where appropriate. Keep everything aligned properly.
Synthesize the complementary DNA sequence.
B. Transcribe the mRNA sequence starting with the 5’ end on the left-hand side.
C. Write the complementary tRNA sequence for each reading frame.
D. Use the genetic code table from our lecture notes or from our textbook (Bauman, 4th ed., p. 208; Bauman, 3rd ed, p. 209; Bauman, 2nd ed., p. 211) to translate the amino acid sequence of the encoded polypeptide, using the three-letter code for each amino acid. Note: Use only the 20 amino acids that are most commonly found in all living organisms.
3’ 5’
1. CCC GAC TCA CTT TAG CCG TTA TAG GGA AAA ATC
A.
B.
C.
D.
5’ 3’
2. GGT GCG CAC ATT TAC AAA GTT AAC CTC CAG GTA
A.
B.
C.
D.
(micro biology assginment)
Explanation / Answer
1. 3' CCC GAC TCA CTT TAG CCG TTA TAG GGA AAA ATC 5'
A. 5' GGG CTG AGT GAA ATC GGC AAT ATC CCT TTT TAG 3' - Complementary DNA sequence
B. 5' GGG CUG AGU GAA AUC GGC AAU AUC CCU UUU UAG 3' - mRNA sequence
C. 3' CCC GAC UCA CUU UAG CCG UUA UAG GGA AAA AUC 5' - complementary tRNA sequence
D. Gly- Leu- Ser- Glu- Ile- Gly- Asp- Ile- Pro- Phe- Stop codon - Amino acid sequence
2. 5' GGT GCG CAC ATT TAC AAA GTT AAC CTC CAG GTA 3'
A. 3' CCA CGC GTG TAA ATG TTT CAA TTG GAG GTC CAT 5'
B. 5' GGU GCG CAC AUU UAC AAA GUU AAC CUC CAG GUA 3'
C. 3' CCA CGC GUG UAA AUG UUU CAA UUG GAG GUC CAU 5'
D. Gly- Ala- His- Ile- Tyr- Lys- Val- Asp- Leu- Glu- Val
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