16. You lsolate a gene that you think could be Important for blood vessel format

Question

16. You lsolate a gene that you think could be Important for blood vessel formation. After you isolate it and put in bacteria, you sequence it (below) to better understand ts tuncto."... a. Identify the first 10 amino acids. 17. A fragment of a wild-type protein contains the following nine amino acids: N... Trp-Trp-Trp-Met-Arg-Glu-Trp-Thr-Met... In four mutant versions of this protein, this same fragment contains the following amino acids: Mutant #1 N. Trp-Trp-Trp-Met-c Mutant #2 N. . Trp-Trp-Trp-Met-Arg-Asp-Trp-Thr-Met C Mutant #3 N. . Trp-Trp-Trp-Met-Arg-Glu-Trp-Met-Met. C Mutant #4 N Trp-Tr-Trp-Met-Arg-Glu-Met-Asp-Asp c Each mutant protein is the consequence of a single nucleotide change: either a substitution of one nucleotide, an insertion of one nucleotide, or a deletion of one nucleotide a) Using the information provided by the mutant protein sequences (and the codon table in your textbook), deduce the sequence of the wild-type mRNA

Explanation / Answer

16. The answer is

GTC GGT CCA GCA CTT CTG TCT GGC GAT GGC AAA GAC ATC TTT TGC GCT TTT ACT CCT CGA CAT CCT CCT CAC CGT CAA CGT GGC TAA AGC GAT A - DNA
CAG CCA GGU CGU GAA GAC AGA CCG CUA CCG UUU CUG UAG AAA ACG CGA AAA UGA GGA GCU GUA GGA GGA GUG GCA GUU GCA CCG AUU UCG CUA U -mRNA

1 GTC GGT CCA GCA CTT CTG TCT GGC GAT GGC AAA GAC ATC TTT TGC 45
1 Val Gly Pro Ala Leu Leu Ser Gly Asp Gly Lys Asp Ile Phe Cys 15

46 GCT TTT ACT CCT CGA CAT CCT CCT CAC CGT CAA CGT GGC TAA AGC 90
16 Ala Phe Thr Pro Arg His Pro Pro His Arg Gln Arg Gly End Ser 30

91 GAT 93
31 Asp

The frist ten amino acids are
Val Gly Pro Ala Leu Leu Ser Gly Asp Gly

ACCORDING TO CHEGG GUIDELINES WE HAVE TO ANSWER ONE QUESTION AT A TIME. POST THE REST AS SEPERATE QUESTIONS THEN I CAN HELP YOU.

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