4. You have isolated a yeast protein involved in transcriptional regulation and

Question

4. You have isolated a yeast protein involved in transcriptional regulation and found a stretch of basic amino acids in the middle of this protein. You suspect that this sequence is a nuclear localization signal. A. What experiment would demonstrate that this sequence is necessary for nuclear targeting of this protein? Describe the experiment in one sentence, and indicate the expected outcome in the second sentence. (4 points)

Experiment:

Expected outcome:

B. What experiment would demonstrate that this sequence is sufficient for nuclear targeting of this protein? Describe the experiment in one sentence, and indicate the expected outcome in the second sentence. (4 points)

Experiment:

Expected outcome:

Explanation / Answer

1) tag this protein with GFP and remove the stretch of this amino acid sequence and express it. find its location with the help of GFP tag.

the fluorescent will visible in the cytoplasm.

2) In another set of experiment insert the stretch of this amino acid sequence along with GFP tag in other protein and express it. find its location with the help of GFP tag.

the fluorescent will visible in nucleus.

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