1- Which enzymes do bacteria produce naturally as a defense against foreign DNA

Question

1- Which enzymes do bacteria produce naturally as a defense against foreign DNA injected into the bacteria by virus?

A- Ligas.

B- Restriction enzymes.

C- DNA polymerase.

D- Antibiotics.

2-After transformation of E. coli with pGLO, why are the cells grown in LB broth for 20 minutes before plating on Amp plates?

A- The pGLO plasmid does not fully enter the E. coli cells until 20 minutes after the heat shock.

B- The Arabinose promoter needs at least 20 minutes before RNA polymerase can bind to the promoter.

C- The Green Fluorescent Protein takes -20 minutes to be transcribed and translated.

D- There is a phenotypic lag of -20 minutes before the b lactamase gene for Amp resistance can be expressed.

Explanation / Answer

!. Restriction Enzyme

2. There is a phenotypic lag of -20 minutes before the b lactamase gene for Amp resistance can be expressed.

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