1- What is PCR and 2- PCR is a simplified version of what organism DNA replicati

Question

1- What is PCR and 2- PCR is a simplified version of what organism DNA replication 3- What is the chance of random matching of two individual DNA profile 4- Who, when (year) and where developed PCR technique. 5- What are the three stages of a PCR cycle, function, temperature (use drawing) 6- What is "taq" Where it come from, What is the reason that has been used in PCR technique? 7- Define following CDNA RT PCR Real time PCR 8- Explain (drawing) why PCR needs 3 cycles to form PCR target products 9- Component of PCR reaction 10- Real word application of DNA

Explanation / Answer

1. Polymerase chain reaction.

2. Prokaryotic organism

3. 1 in 13 billion.

4. KARY MULLIS IN 1983.CETUS CORPORATION

5. Denaturation for opening the two strands of DNA. (96 degrees)

Annealing (55- 65 degrees)- For binding of primers.

Extension (72 degrees)- For the further synthesis of DNA.

6. Taq stands for Thermus aquaticus.

It is a polymerase which can work at high temperatures and helps in the polymerization of DNA.

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