lease give a detailed explanation of the Figure based on the article and not the
ID: 215978 • Letter: L
Question
lease give a detailed explanation of the Figure based on the article and not the figure summary given in the article. I would like to understand the significance of the figures 1-3 ( please explain in you own wordsj and in detailed please) but of course use appropraite vocabulary when referring to certain genes, enzymes, etc to understand the thought process. Thank you.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC16259/ (link to free article)
If article is cited then an online souce can be posted.
Cited Reference: Andrechek Eran R, Amplification of the neu/erbB-2 oncogene in a mouse model of mammary tumorigenesis
In lab a student isolated "Protein X" which he believes is a DNA helicase with 3-5'polarity. The following reagent (see diagram below) is given in lab and the student is asked to design an experiment to determine if this is the case. This reagent is a single stranded piece of circular DNA annealed to a 250-base radiolabeled DNA with a restriction site for the enzyme Smal. restriction enzyme cleavage site 3 5' 200 50 bases bases Please describe the experiment you would perform and include the results you would expect if experimental design.Explanation / Answer
The researchers intend to understand the mechanism behind transcriptional and translational control of gene neu and breast cancer development in mice secondary to the control of endogenous promoter. In order to do so, they constructed a genetically modified mice model in which they intentionally modified the promoter region so that the expression of gene neu could be controlled and hence the protein content of Neu could be managed.
The figure 1 shows a diagram demonstrating the complete construct of the gene for manipulation. Homozygous knock-out and heterozygous knock-out and normal mice were then assessed for the expression levels of the target DNA using Southern blotting demonstrating the expression pattern of single and both alleles in homozygous and heterozygous conditions, respectively.
The figure 2 shows a diagram in which the total construct design of the genes is shown using representation in order to generate a transgene under viral promoter control. The othe parts of figure 2 shows RNase protection assay for different tissues of the same mice using different probes against the target gene.
The figure 3 shows the construct diagram in which a selectable marker for neomycin resistance was added along with EcoRI restriction site. This construct and its effect of different tissues of the same mice was then analysed using Southern blotting.
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