cellular biology wiz needed 5) Serine 795 (S795) of Rb is phosphorylated by CDK4

Question

cellular biology wiz needed

5) Serine 795 (S795) of Rb is phosphorylated by CDK4, and this leads to Rb inactivation. Suppose you want to study the effects of persistent phosphorylation of Rb on Serine 795. You cannot easily regulate the phosphorylation of Serine 795, so you instead need to generate a mutation in the Rb gene that makes Serine 795 always appear to be phosphorylated. The codon that encodes for S795 in Rb is TCA. How could you alter S795 in Rb so that the cell thinks that it is always phosphorylated? Explain your answer. Answer in no more than 2 sentences. (4 points) a) b) How would you mutate the codon that encodes for S795 (TCA) in Rb to achieve your goal for a)? (1 point)

Explanation / Answer

Ans-A to make the S795 in such a condition that the cell thinks it is always phosphoryated it has to be mutated by any type of mutagen. The codon TCA may be changed like TAC, ATC or CTA.

Ans-B we can use some mutagen which may alter the codon sequence. The alteration in codon sequence will automatically generated mutation. The acid of phosphorus may be used to phosphorylation.

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