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1. Biotechnology can be defined as the use of microorganisms, cells, or cell com

ID: 214386 • Letter: 1

Question

1. Biotechnology can be defined as the use of microorganisms, cells, or cell components to make a product, like wine, cheese, vitamins, etc. Recombinant DNA technology is best defined as
A. Natural selection
B. Insertion or modification of genes to produce desired proteins
C. Self-replicating DNA
D. Site-directed mutagenesis
E. Combining proteins in a lab to make new species

2.Natural selection is best defined as a concept in which
A. Man chooses the best features of different animals, plants or microbes to reproduce
B. The environment chooses organisms with characteristics that enhance survival and are therefore more likely to reproduce
C. There are predictable winners and losers in competition even though all organisms will equally reproduce
D. Mutations dictate which organisms will have advantages or disadvantages and man can choose which features are best for biotechnology
E. Darwin tried to justify artificial selection in pea breeding programs

3. Restriction enzymes
A. Are used to cut DNA at specific sequences
B. Can only create matching sticky ends
C. Are sometimes used to remove DNA from surgical instruments
D. All of the above are correct
E. None of the above are correct

4. Which of the following is NOT an important feature for vector usage?
A. Chain reactions
B. Replication
C. Preservation
D. Size
E. Selection markers

5. PCR is a laboratory process. Which of the following are the correct steps in order?
A. Bond primers, separate original DNA strands, DNA elongation
B.   Separate original DNA strands, bond primers, DNA elongation
C. Separate original DNA strands, DNA elongation, bond primers
D. DNA elongation, bond primers, separate original DNA strands
E. Bond primers, DNA elongation, separate original DNA strands

6. Gene silencing depends on which of the following?
A. mRNA is destroyed by restriction enzymes
B.   A siRNA binds to an mRNA transcript which tags it to be destroyed by RISC
C. tRNAs are destroyed by siRNA and therefore mRNA is never translated
D.   siRNA binds directly to DNA and prevents the process of transcription
E. A mRNA binds to a tRNA instead of a ribosome



Explanation / Answer

1. (B) Recombining the genes in the DNA to produce desired proteins.

2. (B) The environment chooses organisms with characteristics that enhance survival and are therefore more likely to reproduce.

3. (A) Are used to cut DNA at specific sequences.

4. (A) Chain reactions.

5. (B) Separate original DNA strands, bond primers, DNA elongation .

6. (B)  A siRNA binds to an mRNA transcript which tags it to be destroyed by RISC.