5) A novel allele (called HbX) has been discovered for the HBB gene. The HbX all

Question

5) A novel allele (called HbX) has been discovered for the HBB gene. The HbX allele has a mutation at amino acid residue # 15 that changes a methionine to glutamic acid. The structure of these two amino acids is shown below. Indicate on the gel on the previous page where you think this novel form of hemoglobin would migrate to if it were loaded into the last (empty) well. Assume the hemoglobin is from an individual that is homozygous for the HbX allele. N-C-C OH CH 9% H2 o O Methionine Glutamic acid 6) Below is a karyotype for Individual #3 from the gel on the previous page. Using the following allele symbols, note the alleles present and the locations for both the alpha and beta subunit genes. Allele symbols: HBA1 - normal alpha subunit allele; HBB- normal beta subunit allele; HBS - sickle cell allele. Some symbols may be used twice or not at all. 10 7) How much agarose would you need to prepare 150 ml of a 1.0% agarose solution? a) 0.15g b) 0.75g c) 1.5g d) 75g

Explanation / Answer

7. Percentage = Agarose quanity in grams /Total qunaity of solution * 100.

Agarose quanity= Percentage x Total qunaity of solution / 100.

Agarose quanity = 1.0 *150/100 = 1.5 grams.

So, the answer is C) 1.5 g.

For 5 and 6 questions, the gel image is required and ACCORDING TO CHEGG GUIDELINES WE HAVE TO ANSWER ONE QUESTION AT A TIME. POST THE REST AS SPERATE QUESTIONS, THEN I CAN HELP YOU.

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