48. What is the correct order of transcriptional events occurring after RNA poly
ID: 214271 • Letter: 4
Question
48. What is the correct order of transcriptional events occurring after RNA polymerase binds to the promoter?
a) start of RNA synthesis, open complex formation, closed complex formation, promoter clearance
b) closed complex formation, open complex formation, promoter clearance, start of RNA synthesis
c) open complex formation, closed complex formation, start of RNA synthesis, promoter clearance
d) open complex formation, closed complex formation, promoter clearance, start of RNA synthesis
e) closed complex formation, open complex formation, start of RNA synthesis, promoter clearance
49. Which of the following is not true of the kinetic proofreading reaction of bacterial RNA polymerase?
a) rNTP is the product.
b) It occurs when the enzyme stalls after incorporating a mismatched base.
c) The type of reaction is pyrophosphorolysis.
d) Water is the nucleophile.
e) Pyrophosphate is a reactant.
.
50. Which of the following RNA products are least likely to be synthesized by RNA polyinerase Il eukaryotic cells?
a) noncoding RN As
b) microRNA
c) mRNA
d) tRNA
e) None of the choices given is a RNA polymerase Il product.
51. Which of the following statements regarding transcription only applies to prokaryotes and not to eukaryotes?
a) A region of the promoter about 30 nucleotides upstream of +1 contains AT-rich base pairs.
b) The RNA start site is designated +1.
c) A region of the promoter about 10 nucleotides upstream of +1 contains AT-rich base pairs.
d) Important sequence elements are frequently found downstream of +1.
e) All of the choices given apply to eukaryotes.
52. Which of one the following amino acids is not phosphorylated in the C-terminal domain of the RPBI subunit of RNA polymerase Il?
c) Tyr
d) Thr
e) All of the amino acids given are phosphorylated.
Explanation / Answer
48.e)))First initiation reaction by forming a closed binary complex.The closed complex is converted to ab open complex by melting short region of DNA within the sequence.Then start of RNA synthesis takes place which contains RNA,DNA and protein.Then promoter clearance takes place.
49)a))rNTP is not the product of bacterial proofreading reaction of RNA polymerase.
50))a))Non coding RNA is least likely product of RNA polymerase II.
51))c) A region of promoter about 30 nucleotide upstream of +1 contain AT rich sequence.These elements only function in double stranded DNA.The -10 sequence has the consensus YATAYAT.
52))In this question i am not able to see the a and b option hence i cant answer this part.
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