Bacteriophage T7 (a virus that infects E. coli) has a sedimentation coefficient,
ID: 213948 • Letter: B
Question
Bacteriophage T7 (a virus that infects E. coli) has a sedimentation coefficient, s = 453 S and a diffusion coefficient, D = 6.03 x 10-8 cm 2 s -1 . (T = 20 o C) The density of the virus is 1.55 g/mL.
B) It can be calculated from phosphorus and nitrogen analyses of the phage that 51.2% (by weight) of the phage is DNA (the rest of the mass is protein, water, salts, etc.). Each phage contains only one DNA double helix. Calculate the molecular weight of the DNA. Each nucleotide has an average molecular weight of 1 Basepair = 660 g/mole; use that information to calculate the number of DNA base pairs in the T7 virus.
Explanation / Answer
T7 virus DNA moleculer weight is 40kbp.
1 basepair=660 g/mole
40kilo basepair=?
=660*40 g/mole
=26400 g/mole
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