You isolate virus particles and resuspend them in 2 ml of a buffered solution co
ID: 213267 • Letter: Y
Question
You isolate virus particles and resuspend them in 2 ml of a
buffered solution containing a total of 6 × 10^9
latex beads.After doing laborious and careful dilutions, shadowing, and
other things necessary for electron microscopic examination,
you view a number of equal fields and determine that you
have 3 beads for every 9 virions. What is the approximate
number of virions present in each milliliter of your beginning
stock solution?
1.B
You isolate virus particles and resuspend them in 5 ml of a
buffered solution containing a total of 30 × 10^9 latex beads.
You view a number of equal fields and determine that you
have 2 beads for every 14 virions. What is the approximate
number of virions present in each milliliter of your beginning
stock solution?
Explanation / Answer
A.
Total number of beads = 6 X 10^9
Number of virions per bead = 9/3 = 3
Total number of virion particles = 6 X 10^9 X 3
= 18 X 10^9 virion particles/2 mL
= 9 X 10^9 virion particles/mL
A.
Total number of beads = 30 X 10^9
Number of virions per bead = 14/2 = 7
Total number of virion particles = 30 X 10^9 X 7
= 210 X 10^9 virion particles/5 mL
= 210 X 10^9 virion particles/5 mL
= 4.2 X 10^10 virion particles/mL
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