Motility Determination (contnued 3. Differentiate between the following types of

Question

Motility Determination (contnued 3. Differentiate between the following types of movement observed in a wet mount or hanging drop a. directional moility b. Brownian movement c. water current movement 4 Between wet mount and hanging drop slide preparations, which is more resistant to evaporation works best with phase-contrast microscopy? What concentration of agar is used in a semisolid medium for motility determination? How does that compare to a typical solid medium (see Exercise 19)? Explain. 5. 6. Why are semisolid media sometimes preferred over slide techniques for evaluating bacterial motility? 7. If SIM medium was used for motility determination for Proteus vulgaris, what noticeable change to the medium was observed? (Hint: See Exercise 41 to find out what the letters "SIM" represent.) 8. Which culturing method provided the clearest results, the semisolid medium tube or the soft agar plate? Explain.

Explanation / Answer

3. Directional motility is assisted by flagella. Bacteria moves by its will in a specified direction.

Brownian movement is movement of particles or bacteria due to the bombardment of surrounding particles in a random manner

Water current movement is makes the particles or bacteria to move along with the current.

4. Wet mount preparation is more resistant to evaporation compared to hanging drop. Wet mount is more suitable for phase contrast microscopy.

5. 0.5% agar is used to prepare semi solid agar medium which helps to determine motility of bacteria. Normally 2% agar is used for culture media.

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