assignment A genetic laboratory has created a series of different E. coli strain

Question

assignment

A genetic laboratory has created a series of different E. coli strains with temperature-sensitive mutations in proteins involved in DNA replication. Based on the phenotype of the mutants, give an indication of which function or enzymes are affected by the mutations:
a. Okazaki fragments accumulate in the bacterium, and the DNA synthesis is not completely completed.
b. No priming of DNA synthesis is observed
c. Synthesis is exclusively distributive
d. DNA supercoiling accumulates in the circular genome, and the DNA synthesis does not run to an end.
e. Newly synthesized DNA strands contain many "mismatched" nucleotides, i.e. non-Watson-Crick base pairs.

Explanation / Answer

Replication occurs as follows

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