3/19/2018 06:00 PM 91.9/100 Gradebook Print Calculator Periodic Table Question 1

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3/19/2018 06:00 PM 91.9/100 Gradebook Print Calculator Periodic Table Question 14 of 17 Sapling Learning Bacteria and other microbes can be used to "clean up" an oil spill by breaking down oil into carbon dioxide and water. Two samples isolated from the Deepwater Horizon leak in the Gulf of Mexico were labeled A and B. The DNA of each was isolated and the percent thymine measured in each sample. Assume the organisms contain normal double-stranded DNA and predict the composition of the other bases Sample A contains 17.1% Thymine Sample B contains 28.3% Thymine Number Number %A Number Number %G %, G Number Number %C %C Both samples are then denatured to remove the secondary structure. Which will have the higher temperature to denature? Previous Give Up & View Solution Check Answer Next Exit Hint

Explanation / Answer

Sample A contains:

17.1 % Thymine

17.1 % A

32.9 % G

32.9 % C

Sample B contains:

28.3 % Thymine

28.3 % A

21.7 % G

21.7 % C

Sample A will have the higher temperature to denature. Sample A have the higher GC region in compare to sample B. GC base pairs are stronger than AT because they have three hydrogen bonds. So, they need higher temperature to denature.

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