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3 attempts left Check my work Enter your answer in the provided box. Given two c

ID: 211286 • Letter: 3

Question

3 attempts left Check my work Enter your answer in the provided box. Given two complementary strands of DNA containing 1.01 x 104 base pairs each, calculate the ratio of two separate strands to hydrogen-bonded double helix in solution at 3.25 x 102 K. (Hint: The formula for calculating this ratio is eAEIRT, where AE is the energy difference between hydrogen- bonded double-strand DNAs and single-strand DNAs and R is the gas constant.) Assume the energy of hydrogen bonds per base pair to be 7.70 x 102 kJ/mol under laboratory conditions. This model is a simplification of reality. References Multipart Answer Difficulty: Hard

Explanation / Answer

we will use the formula to calculate the ratio of two separate strand to hydrogen bonded double helix in solution at 3.25 * 102K.

number of separate DNA strand / number of hydrogen bond of double helix = e E/RT

E = energy difference between hydrogen bonded DNA helix and single stranded DNAs

R = universal gas constant

T = temperature at kelvin

Given that,

number of bases in each single stranded of DNA = 1.01 * 102

energy of hydrogen bond per base pair = 7.70 *10-2KJ/mol

therefore, E = 1.01*102(7.70*10-2) = 7.777 KJ/mol