1. You are studying a new species of microorganism that was discovered at the bo
ID: 211101 • Letter: 1
Question
1. You are studying a new species of microorganism that was discovered at the bottom of the ocean and appears to have been isolated from other species for millions of years. You begin analyzing the species and you realize that it does still use the same four bases of DNA and RNA that are found in the rest of living organisms you know this because you sequenced the DNA that encodes several proteins. However, when you begin to analyze its proteins, you reali that none of the proteins contain the amino acid Alanine, only the 19 other common amino acids. Assume that the organism uses the standard triplet code in explaining your answers. A. What are two possible explanations for why none of the proteins have the amino acid Alanine present? B. How would you determine whether either of these two possibilities are likely to be correct? If possible, please describe the experiment and include the experimental set-up used by Nirenberg and Leder if possible to test one of your hypotheses. Assume you can isolate the ribosomes and charged tRNAs from this species and specifically radiolabel each amino acid.Explanation / Answer
A.
1.During translation t RNA transfer amino acid to ribosomal site. Usually 20 type of amino acid as well as 20 t RNA are found in a cell. But if any particular t RNA is absent then it will be altogether absent in all proteins. For example alanine t RNA may be absent.
2.genes present in that organism do not have colons for alanine.
3. The organism modified it self to adapt it self according to it environment where alanine is not suitable due to some specific reasons hence eliminated with due course of time.
B. Isolated all the tRNA and subjected to Nirenberg and lederhosen experiment as follows
this experiment is called as ribosome binding assay. Several combinations of mRNA were made and passed through a filter which contained ribosomes. Unique triplets codon combination promoted the binding of specific tRNAs to the ribosome. By associating the tRNA with its specific amino acid, it was possible to determine the triplet mRNA sequence that coded for a specific amino acid such as alanine.
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