3. Draw line through where the enzyme EcoRI will cut this sequence. What a kind

Question

3. Draw line through where the enzyme EcoRI will cut this sequence. What a kind of end does it produce (sticky/blunt)? (1 point) 5'-TAGCCAAGAATTCTGAAAGG-3 3'-ATCGGTTCTTAAGACTTTCC-5' Draw a line where the enzyme HindllI will cut this sequence. What kind of end does it produce (sticky/blunt)? (1 point) 4. 5'-ACCGTTAAGCTTGCCAATTC-3' 3'-TGGCAATTCGAACGGTTAAG-5' Draw a line where the enzyme Alul will cut this sequence. What kind of end does it produce (sticky/blunt)? (1 point) 5. 5'-ACCTTTAAGCTATTGGGACA-3 3'-TGGAAATTCGATAACCCTGA-5'

Explanation / Answer

[3. Draw line through where the enzyme EcoRI will cut this sequence. What a kind of end does it produce (sticky/blunt)? (1 point) 5-TAGCCAAG*AATTCTGAAAGG-3

3-ATCGGTTCTTAA*GACTTTCC-5

It recognizes site GAATTC. It will cut between G& A. It will produce sticky ends.

Draw a line where the enzyme HindllI will cut this sequence. What kind of end does it produce (sticky/blunt)? (1 point) 4. 5-ACCGTTA*AGCTTGCCAATTC-3

3-TGGCAATTCGA*ACGGTTAAG-5

It recognizes site AAGCTT. It will cut between A& A. It will produce sticky ends.

Draw a line where the enzyme Alul will cut this sequence. What kind of end does it produce (sticky/blunt)? (1 point) 5. 5-ACCTTTAAG*CTATTGGGACA-3

3-TGGAAATTC*GATAACCCTGA-5]

It recognizes site AGCT. It will cut between G& C. It will produce BLUNT ends.

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