During your summer months, to escape the heat of Davis, you work with a group of
ID: 210776 • Letter: D
Question
During your summer months, to escape the heat of Davis, you work with a group of scien- tists at the North Pole to study the bacterium E. luminescent. This is a bacterium that lives in dark icy waters. Your colleagues have found that during certain times of the year, large populations of E. luminescent glow. While you were away at school, your colleagues identi- fied a signal peptide that when introduced to a population of E. Luminescent in the lab, will cause the bacterium to start glowing. Here are the notes they have written for you to bring you up to speed. The signal peptide is produced by E. Luminescent. The signal peptide can then exit the cell and enter a neighboring E. Luminescent. Thus, overtime, an entire population of E. Lumines- cent will come into contact with the signal peptide. Upon contact with the signal peptide, the bacteria produce a luminescent protein Glow Bright (GB). The production of GB can be up- regulated by self-production of the signal peptide. Signal Peptide Gene Circuit Phyenotype ONDOR-X MDDDD high UKOOOOK- o Univenity of California. Davis MADONDOK - -Explanation / Answer
3. If the repressor becomes mutated, it can longer bind with the RNA polymerase. As a result mRNA which encodes signal molecule can easily transcribed and signal molecule also formed. Thus bacteria become able to produce a luminescent protein Glow Bright (GB) and bacteria start glowing.
4. If the activator becomes mutated it can no longer attached with Glow Bright (GB) protein. As a result RNA polymerase cannot move over the DNA and does not produce signal peptide as well. Thus the entire population of E. Luminescent will never come into contact with the signal peptide. So the bacteria do not glow.
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