You are cloning a gene from Candida albicans that you want to express in E. coli

Question

You are cloning a gene from Candida albicans that you want to express in E. coli for purification. You want the E. coli cells to make a protein with the exact amino acid sequence as in Candida albicans. How could you mutate the DNA sequence of your gene of interest to ensure that the 5-CUG-3 codon codes for serine for instead of leucine?

Check all that apply.

change every in-frame 5'-CUG-3' to 5'-UCG-3' change every in-frame 5'-CUG-3' to 5'-UCC-3' change every in-frame 5'-CTG-3' to 5'-TCC-3' change every in-frame 5'-CUG-3' to 5'-AGU-3' change every in-frame 5'-CTG-3' to 5'-TCG-3' change every in-frame 5'-CTG-3' to 5'-AGT-3'

Explanation / Answer

The options that applies are-

change every in-frame 5'-CTG-3' to 5'-TCC-3'

change every in-frame 5'-CTG-3' to 5'-TCG-3'

change every in-frame 5'-CTG-3' to 5'-AGT-3'

We should keep in mind that we are inserting gene of interest and that is a DNA. And DNA contains no uracil rather it contains thymine. We need to mutate DNA to get CTG codon in a manner that we can get serine.

TCC, TCG and AGT will give UCC, UCG and AGU codons respectively and they will all code for serine .

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