Both questions a. True b. False Suppose there is a mutation in the lac operator

Question

Both questions a. True b. False Suppose there is a mutation in the lac operator preventing the lac repressor from binding. Which of the following situations will result in activated transcription? 9. a. Low glucose, low lactose b. High glucose, low lactose c. High glucose, high lactose d. A and C e. B and C 10. You have introduced a mutation into AraC that causes it to lose its ability to bind arabinose. How will this affect the araBAD operon? a. The operon will always be transcribed b. The operon will never be transcribed c. The operon will be regulated normally d. Glucose will now regulate the operon e. Lactose will now regulate the operon Ask a l 11. Imagine that you discover a bacterial operon involved in the synthesi nn is regulated by a repressor protein that binds to an operator ,1) 271 tu

Explanation / Answer

Q.9 Answer A

Low glucose, low lactose

As glucose prevents adenly cyclase from creating cAMP which then binds to CAP which helps the RNA polymerase for transcription, hence transcription is activated.

Q.10 Answer is B

The operon will never be trascirbed. As AraC works as activator in the presence of arabinose and as an inhibitor in the absence of arabinose. And the stated type of mutation which cause the mutational changes on the binding of Ara C to arabinose will hamPer the whole process of operon transcription.

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