PART 1: PROMOTERS, ACTIVATORS AND REPRESSORS o understand gene regulation you mu
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PART 1: PROMOTERS, ACTIVATORS AND REPRESSORS o understand gene regulation you must first understand the role of various proteins involved in regulating the expression of genes. Look back at your central dogma discussion. In that dis- cussion you learned all the parts of a gene and where these parts are located in relation to each or example, a gene will start with a promoter sequence followed by a transcription start site and end with a transcription stop site. Transcription (or gene expression) initiation involves RNA polymerase binding to the pro- moter sequence. However, not every promoter will readily bind RNA polymerase. What influ- ences RNA polymerase binding to the promoter? Promoter strength. The strength of the promoter (number of transcripts produced/time) is mentally. Strong promoters make lots of transcripts, whereas weak promoters make very few transcripts The model below shows the regulation of a gene in E. coli. RNA polymerase binds to the promoter sequence upstream of the region to be transcribed. Without any transcription factors, the basal level of transcript produced by this gene is 5%. DNA Amount of transcript (compared to maximum) 5% RNA RNA RNA bn Prom is seun transrsbiw bing from RNpre RN A 1. Would you categorize the promoter above as strong or weak? Explain.Explanation / Answer
1.The promotter is weak as transcription efficiency is weak, because transcription efficiency is weak. Because promoter sequence does not closely resemble the ideal sequence for the polymerase to bimd.
2.TATA box is the DNA sequence determined in the promoter.It is a consensus sequence where a protein binds to DNA for the formation of preinitiation complex of RNA polymerase.
THE single nucleotide change TAAGAT is the mutation that forms alternative transcriptionas factors and lowers the RNA expression.
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