8. (1.5 pts) What would be your prediction if isolated mitochondria were incubat

Question

8. (1.5 pts) What would be your prediction if isolated mitochondria were incubated in a medium with excess NADH in the presence of an uncoupling agent such as dinitrophenol (pp. 272 273)? Choose any answers that apply a. The intermembrane space would be more acidic than the matrix. b. The intermembrane space would more basic than the matrix. c. The intermembrane space pH will be the same as the matrix. d. ATP synthesis would occur continuously e. ATP synthesis would be reduced or stop altogether f. NADH would NOT be oxidized continuously by the electron transport chain g. NADH would be continuously oxidized by the electron transport chain (1.5 pts) If you remove the outer mitochondrial membrane, but leave the inner membrane intact, what experimental treatment could you do to "force" ATP production? 9. 10. (3pts) Electron micrographs show that mitochondria in heart muscle have higher density of cristae that mitochondria in skin cells. Why do you think this is so? in cardiac muscle are immobilized at positions between myofibrils. Why do you think this arrangement might be a functionally important difference between these two cells? 11. (3pts) Mitochondria in liver cells appear to move freely in the cytosol, whereas those

Explanation / Answer

8. What would be your prediction if isolated mitochondria were incubated in a medium with excess NADH in the presence of uncoupling agent such as dinitrophenol
Options:
e. ATP synthesis would be reduced or stop altogether
g. NADH would be continuously oxidized by the electron chain.

Explanation: In presence of an uncoupling agent connection between electron transport and phosphorylation is broken and the electron transport chain is kept on thereby seizing the production ATP.

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