Both please. Thank you! Dilution Worksheet [Compatibility Mode] a Search in Docu

Question

Both please. Thank you!

Dilution Worksheet [Compatibility Mode] a Search in Document Home Insert Design Layout References Mailings Review View + Share A Tahoma Paste Styles Pane Emphisis Heading 1 Sireng Subit organisms per mL. You need to dilute this culture to a concentration of 1 X 10 viable organisms per mL. Diagram how you will do this, and identify the volume of aliquots and diluents needed. 6. You don't always DILUTE, sometimes you are able to CONCENTRATE cells. The original volume of your culture was 1 X 108 viable cells per mL. As part of your experiment, you centrifuge 100 ml of your culture and resuspend those cells in 10 ml. What is the concentration of bacteria in the culture now? 7. When diluting concentrated organisms, serial dilutions of 1:10 or 1:100 are preferable to using fewer larger dilutions, such as 1:1000. Explain why. Page 1 of 1 979 Words English (US) Focus- 225%

Explanation / Answer

6. The initial concentration of the culture is 1 x 108 per ml. Using centrifugal methods, the concentration of the volume of the sample is reduced from 100 ml to 10 ml. So, the concentration of the cell will be

C = Initial concentration x Initial volume / Final volume = 1 x 108 x 100 / 10 = 1 x 109 per ml.

7. Fewer larger dilutions are preferred like 1:1000 (if required). This will save time and the dilutions can be said to be prepared at the same time.

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