Both please! Dilution Worksheet [Compatibility Mode] a Search in Document Home I

Question

Both please!

Dilution Worksheet [Compatibility Mode] a Search in Document Home Insert Design Layout References Mailings Review View + Share A Tahoma Paste Handing1 Styles Pane Emphisis Sireng 4. You made a dilution series, ending with a 10-6 dilution. You plated 1.0 ml, 0.1 ml, and 0.01 ml of the final dilution (difficult to do, but for our purposes, consider it done). And you did it in duplicate. The table below illustrates your results Plate 1 299 25 Plate 2 287 31 Volume Plated 1.0 ml 0.1 ml 0.01 ml Identify the countable plates. Use only the countable plates in your calculations. Calculate the number of organisms in your concentrated original (as organisms/mL) Remember that there is ONLY 1 original concentration. You must average counts for different plates or different dilutions to come up with a single number for the original. 5. You have a culture of Escherichia coli that has a known concentration (based on previous dilution and plating - how lucky that many bacterial cultures can handle refrigeration). That known concentration is 3 X 107 viable organisms per mL. You need to dilute this culture to a concentration of 1 X 103 viable organisms per mL. Diagram how you will do this, and identify the volume of aliquots and diluents needed. Page 1 of 1 979 Words English (US) Focus- 225%

Explanation / Answer

countable plates are once with dilution

Plate 1 25

Plate 2 31

Average = 25+31=56/2=28

28 organisms in 0.1 ml of of 10-6 dilution

280 organisms in 1 ml of of 10-6 dilution

2800 organisms in 1 ml of 10-5  dilution

28000 organisms in 1 ml of 10-4  dilution

280000 organisms in 1 ml of 10-3  dilution

2800000 organisms in 1 ml of 10-2 dilution

28000000 organisms in 1 ml of 10-1 dilution

Organisms in original concentrate 280000000 organisms / 1 ml

Related Questions

Navigate

• Browse (All)
• Browse B
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.