1oop ao not always produce a functional suppressor tRNA due to the existence of

Question

1oop ao not always produce a functional suppressor tRNA due to the existence of a key step that contributes to translational fidelity that does not involve the ribosome. What is this step and how might it prevent suppressor able to incorporate amino acids at stop codons? tRNAs from being F) Are there any other tRNAs for other amino acids that might be converted into suppressor RNAs by point mutations in their anticodon loops? Give one example, n f 5. The rated into a cell's DNA as r.tul.nry alreN volel coic in Figure Figure 1 A. Integrated viral DNA You want to know the structure of the viral genome as it exists in the integrated state. You therefore perform a Southern Blot and obtain the data shown in Figure 2. To do the experiment, you use three different restriction endonucleases to digest viral DNA that was purified from virions (V in Figure 2, below) and DNA purified from cells that contain the integrated virus (C in Figure 2). After you separate the fragments by electrophoresis on an agarose gel, you blot the

Explanation / Answer

Integrated viral DNA acts as a provirus in case of Retrovirus. This viral genome got replicated along with host genome and passed on to offspring. But any changes in the environmental condition of the host may lead to viral transcription and also activate new viruses. During the process of viral integration linear viral DNA enter into the host genome. When virus enter the cell, the RNA genome is reversed.

To determine the structure of Viral genome, southern blot technique is used. It helps to detect the DNA sequence in the specific DNA sample. For this agrose gel is applied to sample and then fragments of DNA got seperated by electrophoresis. Then the gel is placed in salt solution and then absorbent material draw the salt solution and DNA fragment is transfered into filter.

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