2. Make a list, in the correct sequence, of the steps that must be followed to e

Question

2. Make a list, in the correct sequence, of the steps that must be followed to extract DNA from cells 3. What is the main purpose of polymerase chain reaction (PCR)? Why is PCR necessary? 4. What is the main purpose of studying a DNA sample through Gel Electrophoresis? 5. Make a list of the necessary steps to make DNA clones (Molecular cloning). Be sure to place the steps in the correct sequence. 6. What is reproductive cloning? What are some possible applications of reproductive cloning? 7. Explain how gene therapy works. How are genes inserted into a different organism? 2. Make a list, in the correct sequence, of the steps that must be followed to extract DNA from cells 3. What is the main purpose of polymerase chain reaction (PCR)? Why is PCR necessary? 4. What is the main purpose of studying a DNA sample through Gel Electrophoresis? 5. Make a list of the necessary steps to make DNA clones (Molecular cloning). Be sure to place the steps in the correct sequence. 6. What is reproductive cloning? What are some possible applications of reproductive cloning? 7. Explain how gene therapy works. How are genes inserted into a different organism? 2. Make a list, in the correct sequence, of the steps that must be followed to extract DNA from cells 3. What is the main purpose of polymerase chain reaction (PCR)? Why is PCR necessary? 4. What is the main purpose of studying a DNA sample through Gel Electrophoresis? 5. Make a list of the necessary steps to make DNA clones (Molecular cloning). Be sure to place the steps in the correct sequence. 6. What is reproductive cloning? What are some possible applications of reproductive cloning? 7. Explain how gene therapy works. How are genes inserted into a different organism?

Explanation / Answer

2-cells is collected<Breaking the cell membranes open to expose the DNA along with the cytoplasm<

Breaking RNA by adding an RNase

The solution is treated with concentrated salt solution to make debris such as broken proteins, lipids and RNA to clump together<Centrifugation of the solution, which separates the clumped cellular debris from the DNA.<DNA purification from detergents, proteins, salts and reagents used during cell lysis step.

3-The polymerase chain reaction (PCR) is used to amplify DNA samples so there are enough copies.it continuously produces countless copies of DNA sequences in the body.It allows us to make many copies of a targeted segment of DNA.

4-To separate DNA fragments based on their size using electricity

5-1. Cloning of a gene involves arrangement of natural or synthetic DNA in the desired order

2. Introducing the genetic construct into recipient cell

3. Producing a clone in which every cell carries copies of the designed DNA

6-Reproductive cloning is a type of cloning which is performed for the purpose of creating a duplicate copy of another organism,Reproductive cloning is a method used to make a clone or an identical copy of an entire multicellular organism.

7-Gene therapy is designed to introduce genetic material into cells to compensate for abnormal genes or to make a beneficial protein. If a mutated gene causes a necessary protein to be faulty or missing, gene therapy may be able to introduce a normal copy of the gene to restore the function of the protein.

genes inserted into different organism...1) Restriction Enzymes cut DNA in a specific place 2) The DNA fragment is inserted into the new cell by the vector. Vectors include plasmids and viruses.

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