Pg 4: cells are diploid and provided to you at ~2 x10^7 cells/ml One unit of a r

Question

Pg 4: cells are diploid and provided to you at ~2 x10^7 cells/ml
One unit of a restriction enzyme is defined as the amount of enzyme required to digest 1 g of DNA in i hour at 37°C. If Jul of the restriction enzyme acl (concentration-20,000 units ml) is able to completely digest your sample of yeast genomic DNA in 2 hours, how much DNA did you have? Use this information to calculate the DNA content per yeast cell. (See bottom of page 4 for the # of cells you were given in your extraction protocol.)

Explanation / Answer

You are using 1 microlitre of the enzyme means 20 units of it. This digests your yeast genomic DNA in 2 hours. 1 unit of the enzyme means that you can digest 1 microgram of DNA with it. So ideally you can digest 20 micrograms of DNA in 1 hour. Since it takes 2 hours for complete digestion, the DNA content probably is 40 micrograms.

The next part requires the amount of cell culture you have taken for digestion. Supposedly, you had taken 1 ml of your culture for digestion. Then in that 1 ml you had 40 micrograms of DNA. Since 1 ml of culture contains 20 million cells, these cells have 40 micrograms DNA. Applying unitary method yields the DNA content per cell as 2 nanograms.

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