1. What are the total number of bacteria in the before mutation original inoculu

Question

1. What are the total number of bacteria in the before mutation original inoculum culture? What are the total number of bacteria in the after mutation original inoculum culture? Show your work on how you achieved these answers.

2. Based on the above images, how does the bacterial metabolic gene contribute to bacterial growth?

3. Explain how Paul could use these results to his advantage to help develop a therapeutic drug against this bacterial organism.

Serial dilution test after mutation Serial dilution test before mutation 1 mL 1 mL Original inoculum Original inoculum 9 mL 1:101:100 1:1000 1:10000 1:100000 1:101:100 1:1000 1:10000 1:100000

Explanation / Answer

Part a-

To count bacterial we would take 1:1000000 dilution in non-mutation case, while in non-mutation case we would take1:1000 dilution.

In without mutation condition there are 3 colonies in 1:1000000 dilution. Therefore, the numbers of bacteria would be as following.

Number of bacteria in 1ml of sample = 3 × 106        [here 106 is the dilution factor]

In case of mutation, there are 4 colonies in 1:1000 dilution

So numbers of bacteria= 4 × 103

Partb-

Bacterial metabolic gene if does not get mutation, it makes bacteria to grow enormously.

Part c-

We can make a causative agent like siRNA that can make mutation in such bacteria. The therapeutic drug can apply directly on the bacterial affected region and that will bring mutation in bacteria, which will suppress the growth bacterial.

Related Questions

Navigate

• Browse (All)
• Browse 1
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.