please explain answers 3. You plan to perform an Ames Test using a histidine aux
ID: 207743 • Letter: P
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please explain answers
3. You plan to perform an Ames Test using a histidine auxotroph that has a nonsense mutation in the hisGi gene. Below are three experiments (E1-E3) that you plan to perform plus a control (in parentheses) to compare it with. Choose one prediction (A-C) that you expect to observe in your experiment compared to the on what you know about DNA damage and DNA repair. PLUS, explain why you predict this outcome A. Same number of colonies B. More colonies C. Fewer colonies El-DNA mismatch-causing mutagen (with a water control) . Explain why E2- DNA methylation null mutant (with a wild type control) I)Explain why: . Explain why: E3. Frameshift-causing mutagen (with a water control)Explanation / Answer
E1- DNA mismatch causing mutagen more colonies. it is because to the fact that it is a system for recognizing and repairing erroneous insertion, deletion, and mis-incorporation of bases that can arise during DNA replication and recombination.
E2- DNA methylation null mutants is having same numer of colonies. it is because to the fact that Any mutational event that disrupts the superhelical structure of DNA carries with it to compromise the genetic stability of a cell. The fact that the damage detection and repair systems are as complex as the replication machinery itself highlights the importance evolution has attached to DNA fidelit.
E3- famshift causing mutagen with fewer colonies. it is because to the fact that Recognizing and repairing mismatches and indels is important for cells because failure to do so results in microsatellite instability (MSI) and an elevated spontaneous mutation rate (mutator phenotype). In comparison to other cancer types, MMR-deficient (MSI) cancer has a very high frequency of mutations.
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