1. Suppose the restriction enzyme GsuII binds a site that is 4 nucleotides in le
ID: 207602 • Letter: 1
Question
1. Suppose the restriction enzyme GsuII binds a site that is 4 nucleotides in length. How often (how many base pairs) does this enzyme cut DNA?
Your answer must be numeric.
2. Which of the following statement(s) is(are) true about restriction enzymes?
a.discovered in bacteria
b. cleave the phosphodiester bonds in the backbone
c. cleave the peptide bonds in the backbone
d.produce random fragments
e. recognize specific sequences
3.In a DNA library, each plasmid can contain more than one gene. This is not true for a cDNA library because?
a. one molecule of cDNA contains one gene
b. different restriction enzymes are used
c. host cells cannot be transformed when there is more than one gene in the plasmid
d. the vectors used in cDNA are different
4.You wish to create a transgenic mouse that is completely hairless. You introduce the appropriate mutation into embryonic stem cells and implant those cells into early embryos. Those embryos are placed in a surrogate mouse who then gives birth to the first generation of mice. These mice are further bred.
which of the following is (are) true?
a. first generation is completely hairless
b. second generation is completely hairless
c. third generation is completely hairless
d. second geneeration is heterozygous
e. second genertion are crossed with each other
f. first generation are crossed with each other
Explanation / Answer
1. Suppose the restriction enzyme GsuII binds a site that is 4 nucleotides in length. How often (how many base pairs) does this enzyme cut DNA?
The total number of nitrogen bases in DNA (A, T, G and C) are four. So, at a time one can accomidate at one posistion, so the proportion of one base at one position is (Either A or G or T or C) = 1/4.
As the restriction site has 4 nucleotides length, the probability of occurance of the restriction site = 1/4 x 1/4 x 1/4 x 1/4 = 1/256.
The answer is 1/256.
2. Which of the following statement(s) is(are) true about restriction enzymes?
The answers are
a.discovered in bacteria
c. cleave the peptide bonds in the backbone
d.produce random fragments
e. recognize specific sequences
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