5- The movement of a charged molecule subjected to an electrical field directly

Question

5- The movement of a charged molecule subjected to an electrical field directly depends on a. The net charge of the molecule b. The frictional coefficient, mass and shape of the molecule c. The voltage of electric field d. All of the above e. a and c 6- There are two layers of gels in a discontinuous gel electrophoresis method which are characterized as a. The upper layer, a high concentration gel with pH of 6.8 gel b. The lower layer, a low concentration gel with pH of 8.8 c. The upper layer, a low concentration gel with pH of 8.8 d. The upper layer, a low concentration gel with pH of 6.8

Explanation / Answer

The movement of a charged molecule subjected to an electrical field directly depends on all the above.

Explanation - The mobility, or rate of migration, of a molecule increases with increased applied voltage and increased net charge on the molecule. Conversely, the mobility of a molecule decreases with increased molecular friction, or resistance to flow through the viscous medium, caused by molecular size and shape.

There are two Layer of gels in a discontinuous gel electrophoresis meithod which are characterized as - The upper layer, a low concentration gel with pH of 6.8

Explaination

The gel is divided into two parts, resolving and stacking gel, both have different concentrations of polyacrylamide. The one with lower concentration is stacked on top of the one with higher concentration. Glycine has very low net charge at pH 6.8 of stacking gel, so it has low mobility.

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