7- In a sodium dodecy molecules depends on electroporesis GOS PAGE), the movemen

Question

7- In a sodium dodecy molecules depends on electroporesis GOS PAGE), the movement of the protein a. Net charge of molecule b. Shape of the molecule d. All of the above 8. When protein molecules are treated with sodium dodecyl sultate (SDS1, the detergent wn a. increases the molecule mass of the sample b. Binds to hydrophobic region of protein chain and mask the protein with negative charee . Provides a negative net charge in electrophoresis buffer d. increases the frictional coefficient of the protein 9. structure of different organisms, but determine the evolution of Protein and DNA molecules Molecular biology techniques made it possible for Evolutionary Biologist to compare not only the physical a. True b. False .gpts short answer question 1 1. A plot of various wavelength versus their absorption is called 2. and what is its application in molecular biology techniques 3. The wavelength at which a single compound shows the highest absorption is called 4. In SDS-PAGE, why you heated the protein samples prior to electrophoresls 5. Explain the mechanism and the order of movement of the charged molecules in a denaturing SDS-PAE n both stacking and resolving gels.

Explanation / Answer

Ans 7: (d) option is correct that is all the above

Explanation: If two protien have the same mass and shape, one with the greater charge density will move faster through the gel.

If two protien having same charge density and shape, the one of smaller mass or size will migrate faster than the larger size protien.

Shape is also major factor because compact globular protien move more rapidly than elongated fibrous protien of mass.

Ans 8: (c) is correct option that is provides a negative net charge in electrophoresis buffer

Explanation- when protien is mixed with SDS they provide net negative charge. SDS is detergent having a negative charge. It distrupt non covalent bond like hydrogen bond, hydrophobic bond and ionic interaction. SDS binds to main chains at ratio of about one SDS for every two amino acids residue, which imparts a large net negative charge on protien.

Ans 9: True

Explanation- This is true that molecular biology technique made possible for evolutionary biologist to compare not only physical structure of different organisms but determine the evolution of protien and DNA molecule it involve due to PCR, RFLP, AFLP and genomic fingerprint technique. PCR is rapid and versatile method for amplifying defined target DNA sequence present in the source of DNA. In each cycle of reaction, the strand of the duplex DNA are separated by heat denaturation, prepared for cooled to such level tjat synthetic DNA primer anneal to complementary segments on each strand and the primer are extended by DNA polymerase.

Genomic fingerprint use to classify microbes and help to determine phylogenetic relationship. It is not involve in nucleotides sequence. It employ the capicity of restriction endonuclease to recognize specific nucleotides sequence.

The comparison of restriction fragment between species and strain is basis of RFLP.

short type answer

Ans1: Absorption spectroscopy

Ans 2: Application of molecular technique

used in evolution of protien DNA, separation of compound, amplification, diagnosis of diseases

Ans 3: Absorption of ultra voilet and visible region shows highes absorption and the wavelength is 400-200nm and apx 2000 nm.

Ans 4: Heating can cause aggravation of protien.

Related Questions

Navigate

• Browse (All)
• Browse 7
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.