Homework #7 Due Friday March 9,2018 1a. Eukaryotic transcription factors (TFs) (
ID: 206929 • Letter: H
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Homework #7 Due Friday March 9,2018 1a. Eukaryotic transcription factors (TFs) (which are protermene inside the n regulatory sequence of a gene to initiate transcription of a gee which part of the cell the TF proteins are synthesized? (0.5 point (TF in nature) bind to the 1D. In fruit fies, 'GAL4-UAS system is used to study gene express Below is a diagram that shows that when Gal4 proteins b called "UAs present DNA sequence gene X nd at the present in the upstream of the gene X, then transcription from undetectable er level. In the absence of Gal4 proteins. gene X transcript is The gene X has its own promoter "P" downstream of the "UAS" sequence where promoter general transcription factors "TFs can bind. P is the promoter GAL 4 TFs Gal4 gene UAS Increased transcription from gene X From the information, identify what kind of response sequence "UAS is and what kind of regulatory molecule Gal4 proteins are. Explain your answer. (1.5 point)Explanation / Answer
The transcription factors are produced in The DNA of the cell where they are expressed. they are synthesized on the ribosomes as they are proteins. they are proteins that helps in turning off or on specific genes. They regulate transcription of genes.
UAS is upstream activating sequence is a Cis acting element and its presence increases the expression of neighbouring gene. It plays an important role in expression of protein of interest by acting as inducer. The GAl4 -UAE is biochemical method whcih helps in studying gene expression. GAl4 is positive regulator of gene expression in the galactose induced gene.
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